BrightLine®单波段和多波段LED滤光片组 
BrightLine®单波段和多波段LED滤光片组 Semrock从今天市场上受欢迎的led光源的光谱开始，设计这个滤光片系列。与传统的用于标准宽频光源(如水银或氙气灯)的传统滤光片组相比，这些滤光片可显著改善荧光信号。 这些滤光片组被优化，以同时传送亮的信号和高信噪比(对比度)，可用于使用基于LED的光引擎成像各种荧光团。 光谱与流行的LED光引擎对齐 与传统光源相比，在使用led光引擎时，每个通道的信号比传统光源多10倍到100倍 该系列中的常见激发滤光片简化了不同设置配置的可视化 BrightLine Single-band LED Filter Sets BrightLine单波段LED滤光片组 滤光片/主要荧光 | 激发(CWL/BW) | 发射 (CWL/BW) | 二向色边缘 | 滤光片组零件号/ 零组零件编号 | LED-DAPI-A DAPI, Alexa Fluor® 405, BFP, Pacific BlueTM | 392/23 | 447/60 | 409 nm | LED-DAPI-A-000 LED-DAPI-A-000-ZERO | LED-CFP-A CFP (ECFP), Cerulean, SYTOX Blue, TagCFP | 438/24 | 483/32 | 458 nm | LED-CFP-A-000 LED-CFP-A-000-ZERO | LED-FITC-A FITC (Fluorescein), GFP (EGFP), Cy2TM, 5-FAM | 474/27 | 525/45 | 495 nm | LED-FITC-A-000 LED-FITC-A-000-ZERO | LED-YFP-A YFP (EYFP), Venus, Fluo-3, Rhodamine 123 | 509/22 | 544/24 | 526 nm | LED-YFP-A-000 LED-YFP-A-000-ZERO | LED-TRITC-A TRITC, SpectrumOrange, dTomato, mTangerine | 554/23 | 609/54 | 573 nm | LED-TRITC-A-000 LED-TRITC-A-000-ZERO | LED-mCherry-A mCherry, TexasRed, mKate, mRFP1 | 578/21 | 641/75 | 596 nm | LED-mCherry-A-000 LED-mCherry-A-000-ZERO | LED-Cy5-A Cy5TM, Alexa Fluor® 647, APC | 635/18 | 680/42 | 652 nm | LED-Cy5-A-000 LED-Cy5-A-000-ZERO |
“平克尔”多频带LED滤光片组- 单频激发器，一个三或四波段发射器，和一个多分束器 滤光片/主要荧光 | 多通带激发 | 多通带发射 | 多边缘二向色 | 滤光片组零件号 | LED-DA/FI/TR/Cy5-A (Quad-band Full Multiband) Blue: DAPI, BFP, Alexa Fluor® 405 Green: FITC (Fluorescein), GFP (EGFP), Alexa Fluor® 488 Orange: TRITC, DsRed, dTomato, mRFP Red: Cy5TM, APC, Alexa Fluor® 647 | 392/23 474/27 554/23 635/18 | 432/36 515/30 595/31 730/139 | 409 nm 493 nm 573 nm 652 nm | LED-DA/FI/TR/Cy5-A-000 LED-DA/FI/TR/Cy5-A-000- ZERO |
“Sedat”多通带LED滤光片组-单频带激发器和发射器，以及一个多边分束器 滤光片组/主要荧光 | 单波段激发 | 多通带发射 | 多边缘二向色 | 滤光片组零件号 | LED-DA/FI/TX-3X-A (Triple-band Pinkel Set) | | | | LED-DA/FI/TX-3X-A-000 | Blue: DAPI, BFP (EBFP), Alexa Fluor® 405 | Ex1: 392/23 | 432/36 | 409 nm | LED-DA/FI/TX-3X-A-000-ZERO | Green: FITC (Fluorescein), GFP (EGFP),Cy2TM | Ex2: 474/27 | 523/46 | 493 nm | | Red: Texas Red, mCherry, 5-ROX | Ex3: 575/25 | 702/196 | 596 nm | | LED-CFP/YFP/mCherry-3X-A (Triple-bandPinkelSet) | | | | LED -CFP/YFP/mCherry- | Cyan: CFP (ECFP), Cerulean, SYTOX Blue, TagCFP | Ex1: 438/24 | 475/22 | 459 nm | 3X-A-000 | Yellow: YFP (EYFP), Venus, Fluo-3, Rhodamine 123 | Ex2: 509/22 | 543/22 | 526 nm | LED -CFP/YFP/mCherry-3X-A- | Red: mCherry, TexasRed, mKate, mRFP1 | Ex3: 578/21 | 702/197 | 596 nm | 000-ZERO | LED-DA/FI/TR/Cy5-4X-A (Quad-band Pinkel Set) Blue: DAPI, BFP (EBFP), Alexa Fluor® 405 Green: FITC (Fluorescein), GFP (EGFP), Alexa Fluor® 488 Orange: TRITC, DsRed, dTomato, mRFP Red: Cy5TM, APC, Alexa Fluor® 647 | Ex1: 392/23 Ex2: 474/27 Ex3: 554/23 Ex4: 635/18 | 432/36 515/30 595/31 730/139 | 409 nm 493 nm 573 nm 652 nm | LED-DA/FI/TR/Cy5-4X-A-000 LED-DA/FI/TR/Cy5-4X-A-000- ZERO |
“平克尔”多频带LED滤光片组- 单频激发器，一个三或四波段发射器，和一个多分束器 滤光片组/主要荧光 | 单波段激发 (CWL/BW) | 单波段发射 (CWL/BW) | 多边缘二向色 | 滤光片组编号 | LED-DA/FI/TX-3X3M-A (Triple-band Sedat Set) Blue: DAPI, BFP (EBFP), Alexa Fluor® 405 Green: FITC (Fluorescein), GFP (EGFP), Cy2TM Red: Texas Red, mCherry, 5-ROX | Ex1: 392/23 Ex2: 474/27 Ex3: 575/25 | Em1: 433/24 Em2: 525/45 Em3: 641/75 | 409 nm 493 nm 596 nm | LED-DA/FI/TX-3X3M-A-000 LED-DA/FI/TX-3X-A-000-ZERO | LED-CFP/YFP/mCherry-3X3M-A (Triple-band Sedat Set) Cyan: CFP (ECFP), Cerulean, SYTOX Blue, TagCFP Yellow: YFP (EYFP), Eosin, Fluo-3, Rhodamine 123 Red: mCherry, TexasRed, mKate, mRFP1 | Ex1: 438/24 Ex2: 509/22 Ex3: 578/21 | Em1: 482/25 Em2: 544/24 Em3: 641/75 | 459 nm 526 nm 596 nm | LED-CFP/YFP/mCherry- 3X3M-A-000 LED -CFP/YFP/mCherry-3X-A- 000-ZERO | LED-DA/FI/TR/Cy5-4X4M-A (Quad-band Sedat Set) Blue: DAPI, BFP (EBFP), Alexa Fluor® 405 Green: FITC (Fluorescein), GFP (EGFP), Alexa Fluor® 488 Orange: TRITC, DsRed, dTomato, mRFP Red: Cy5TM, APC, Alexa Fluor® 647 | Ex1: 392/23 Ex2: 474/27 Ex3: 554/23 Ex4: 635/18 | Em1: 433/24 Em2: 520/35 Em3: 600/37 Em4: 680/42 | 409 nm 493 nm 573 nm 652 nm | LED-DA/FI/TR/Cy5-4X-A-000 LED-DA/FI/TR/Cy5-4X-A-000-ZERO |
SearchLight允许荧光显微镜用户和光学仪器设计者为他们的显微镜或系统预先确定z佳的荧光素、光源、探测器和光学滤镜组合。通过消除对光谱数据源的猜测和搜索时间，探照灯用户将能够消除误差并更有效的工作。用户可以从大量的预先加载的光谱中选择或者在这个免费且可公开访问的工具中上传他们自己的光谱数据。用户也可以安全地保存和共享数据。 共享:SearchLight的共享功能允许研究人员、工程师、公司和机构之间建立一个*的URL链接，可以通过电子邮件发送给同事或合作者。 立即使用探照灯。节省时间。试一试在:http://searchlight.semrock.com 
Semrock生产多波段荧光滤光片，具有通带、边缘陡度和阻滞性能，可与z佳单波段滤光片相媲美，并且具有硬镀膜的优异“抗烧毁”耐久性。事实上，在每一个BrightLine滤光片组中的滤光片，包括这些多波段的滤光片，都是用同样的耐用的硬镀膜技术制成的。 BrightLine® Multiband Fluorescence Sets BrightLine®多波段荧光滤光片组 滤光片组/主要荧光 | 多波段激发(CWL/BW) | 多波段发射(CWL/BW) | 多边缘二向色 | 滤光片组零件编号 | DA/FI-A (Dual-Band Full Multiband) | | | | DA/FI-A-000 | Blue: DAPI, Hoechst, AMCA, BFP, Alexa Fluor® 350 | 387/11 | 433/38 | 403 nm | | Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor® 488 | 480/29 | 530/40 | 502 nm | | CFP/YFP-A (Dual-Band Full Multiband) | | | | CFP/YFP-A-000 | Cyan: CFP, AmCyan, SYTOX Blue, BOBO-1, BO-PRO-1 | 416/25 | 464/23 | 440 nm | | Yellow: YFP, Calcium Green-1, Eosin, Rhodamine 123 | 501/18 | 547/31 | 520 nm | | GFP/DsRed-A (Dual-Band Full Multiband) | | | | GFP/DsRed-A-000 | Green: GFP, rsGFP, FITC, Alexa Fluor® 488 | 468/34 | 512/23 | 493 nm | | Red: DsRed, TRITC, Cy3™, Texas Red®, Alexa Fluor® 568 & 594 | 553/26 | 630/91 | 574 nm | | FITC/TxRed-A (Dual-Band Full Multiband) | | | | FITC/TxRed-A-000 | Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor® 488 | 479/38 | 524/29 | 505 nm | | Red: Texas Red®, mCherry, Alexa Fluor® 568 & 594 | 585/27 | 628/33 | 606 nm | | Cy3/Cy5-A (Dual-Band Full Multiband) | | | | Cy3/Cy5-A-000 | Yellow: Cy3™, DsRed, Alexa Fluor® 555 | 534/36 | 577/24 | 560 nm | | Red: Cy5™, SpectrumFRed™, Alexa Fluor® 647 & 660 | 635/31 | 690/50 | 659 nm | | DA/FI/TR-A (Triple-Band Full Multiband) | | | | DA/FI/TR-A-000 | Blue: DAPI, Hoechst, AMCA, BFP, Alexa Fluor® 350 | 387/11 | 433/36 | 403 nm | | Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor® 488 | 478/24 | 517/23 | 497 nm | | Orange: TRITC, DsRed, Cy3™, Alexa Fluor® 555 | 555/19 | 613/61 | 574 nm | | DA/FI/TX-B (Triple-Band Full Multiband) | | | | DA/FI/TX-B-000 | Blue: DAPI, Hoechst, AMCA, BFP, Alexa Fluor® 350 | 407/14 | 457/22 | 436 nm | | Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor® 488 | 494/20 | 530/20 | 514 nm | | Red: Texas Red®, MitoTracker Red, Alexa Fluor® 568 & 594 | 576/20 | 628/28 | 604 nm | | DA/FI/TR/Cy5-A (Quad-Band Full Multiband) | | | | DA/FI/TR/Cy5-A-000 | Blue: DAPI, Hoechst, AMCA, BFP, Alexa Fluor® 350 | 387/11 | 440/40 | 410 nm | | Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor® 488 | 485/20 | 521/21 | 504 nm | | Orange: TRITC, DsRed, Cy3™, Alexa Fluor® 555 | 559/25 | 607/34 | 582 nm | | Red: Texas Red®, MitoTracker Red, Alexa Fluor® 568 & 594 | 649/13 | 700/45 | 669 nm | |
高的透射率，阻挡度和边缘陡度，可实现令人眼花缭乱的视觉和数字成像 硬的、绝缘的镀膜为每个滤光片，包括UV激发器的“抗烧毁”性能 光谱复杂的滤光片是我们的特产。世界上仅有的五种彩色多波段组和大量的四、三、双波段组可供选择，也保证有库存。 “平克尔”多波段滤光片组- 单频带激发器，一种双-三-四或五波段发射器和一种多边分束器 滤光片组/主要荧光团 | 多波段激发(CWL/BW) | 多波段发射(CWL/BW) | 多边缘二向色 | 滤光片组编号 | DA/FI-2X-B (Dual-Band Pinkel Set) | | | | DA/FI-2X-B-000 | Blue: DAPI, Hoechst, AMCA, BFP, Alexa Fluor® 350 | Ex1: 387/11 | 433/38 | 403 nm | | Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor® 488 | Ex2: 485/20 | 530/40 | 502 nm | | CFP/YFP-2X-A (Dual-Band Pinkel Set) | | | | CFP/YFP-2X-A-000 | Cyan: CFP, AmCyan, SYTOX Blue, BOBO-1, BO-PRO-1 | Ex1: 427/10 | 464/23 | 440 nm | | Yellow: YFP, Calcium Green-1, Eosin, Rhodamine 123 | Ex2: 504/12 | 547/31 | 520 nm | | GFP/DsRed-2X-A (Dual-Band Pinkel Set) | | | | GFP/DsRed-2X-A-000 | Green: GFP, rsGFP, FITC, Alexa Fluor® 488 | Ex1: 470/22 | 512/23 | 493 nm | | Red: DsRed, TRITC, Cy3™, Texas Red®, Alexa Fluor® 568 & 594 | Ex2: 556/20 | 630/91 | 574 nm | | GFP/HcRed-2X-A (Dual-Band Pinkel Set) | | | | GFP/HcRed-2X-A-000 | Green: GFP, rsGFP, FITC, Alexa Fluor® 488 | Ex1: 474/23 | 527/42 | 495 nm | | Red: HcRed, Cy3.5™, Texas Red®, Alexa Fluor® 594 | Ex2: 585/29 | 645/49 | 605 nm | | FITC/TxRed-2X-B (Dual-Band Pinkel Set) | | | | FITC/TxRed-2X-B-000 | Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor® 488 | Ex1: 485/20 | 524/29 | 505 nm | | Red: Texas Red®, mCherry, Alexa Fluor® 568 & 594 | Ex2: 586/20 | 628/33 | 606 nm | | Cy3/Cy5-2X-B (Dual-Band Pinkel Set) | | | | Cy3/Cy5-2X-B-000 | Yellow: Cy3™, DsRed, Alexa Fluor® 555 | Ex1: 534/30 | 577/24 | 560 nm | | Red: Cy5™, SpectrumFRed™, Alexa Fluor® 647 & 660 | Ex2: 628/40 | 690/50 | 659 nm | | BFP/GFP/HcRed-3X-A (Triple-Band Pinkel Set) | | | | BFP/GFP/HcRed-3X-A-000 | Blue: BFP, DAPI, Hoechst, AMCA, Alexa Fluor® 350 | Ex1: 370/36 | 425/35 | 395 nm | | Green: GFP, rsGFP, FITC, Alexa Fluor® 488 | Ex2: 474/23 | 527/42 | 495 nm | | Red: HcRed, Cy3.5™, Texas Red®, Alexa Fluor® 594 | Ex3: 585/29 | 685/130 | 610 nm | | CFP/YFP/HcRed-3X-A (Triple-Band Pinkel Set) | | | | CFP/YFP/HcRed-3X-A-000 | Cyan: CFP, AmCyan, SYTOX Blue, BOBO-1, BO-PRO-1 | Ex1: 427/10 | 464/23 | 444 nm | | Yellow: YFP, Calcium Green-1, Fluo-3, Rhodamine 123 | Ex2: 504/12 | 542/27 | 521 nm | | Red: HcRed, Cy3.5™, Texas Red®, Alexa Fluor® 594 | Ex3: 589/15 | 639/42 | 608 nm | | DA/FI/TR-3X-A (Triple-Band Pinkel Set) | | | | DA/FI/TR-3X-A-000 | Blue: DAPI | Ex1: 387/11 | 433/36 | 403 nm | | Green: FITC (Fluorescein), GFP (EGFP) | Ex2: 480/17 | 517/23 | 497 nm | | Orange: TRITC (Tetramethylrhodamine) | Ex3: 556/20 | 613/61 | 574 nm | | DA/FI/TX-3X-C (Triple-Band Pinkel Set) | | | | DA/FI/TX-3X-C-000 | Blue: DAPI, Hoechst, AMCA, BFP, Alexa Fluor® 350 Green: FITC, GFP, rsGFP, BoDipy, Alexa Fluor® 488 | Ex1: 387/11 Ex2: 494/20 | 457/22 530/20 | 436 nm 514 nm | | Red: Texas Red®, MitoTracker Red, Alexa Fluor® 568 & 594 | Ex3: 575/25 | 628/28 | 604 nm | | DA/FI/TR/Cy5-4X-B (Quad-Band Pinkel Set) Blue: DAPI, Hoechst, AMCA, Alexa Fluor® 350 Green: FITC, GFP, rsGFP, Bodipy, AlexaFluor® 488 Orange: TRITC, Cy3™, Texas Red®, Alexa Fluor® 568 & 594 Red: Cy5™, APC, TOTO-3, TO-PRO-3, Alexa Fluor® 647 & 660 | Ex1: 387/11 Ex2: 485/20 Ex3: 560/25 Ex4: 650/13 | 440/40 521/21 607/34 700/45 | 410 nm 504 nm 582 nm 669 nm | DA/FI/TR/Cy5-4X-B-000 | DA/FI/TR/Cy5/Cy7-5X-A (Penta-Band Pinkel Set) Blue: DAPI, Hoechst, AMCA, Alexa Fluor® 350 Green: FITC, GFP, rsGFP, Bodipy, AlexaFluor® 488 Orange: TRITC, Cy3™, Texas Red®, Alexa Fluor® 568 & 594 Red: Cy5™, APC, TOTO-3, TO-PRO-3, Alexa Fluor® 647 & 660 Deep Red: Cy7™ | Ex1: 387/11 Ex2: 485/20 Ex3: 560/25 Ex4: 650/13 Ex5: 740/13 | 440/40 521/21 607/34 694/35 809/81 | 408 nm 504 nm 581 nm 667 nm 762 nm | DA/FI/TR/Cy5/Cy7 -5X-A-000 |
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